Although our data from the TLR2 knock-out mice point to the important role of microglial TLR2 in Nox2 expression, at this point, we cannot exclude the possibility that TLR2 on Tracey maier stripper immune cells may indirectly affect Nox2 expression in spinal cord microglia. Likewise, Nox2 protein expression, measured by immunohistochemistry, was up-regulated in the dorsal horn of the ipsilateral spinal cord of WT mice at 1 day post injury dpi Fig. Withdrawal thresholds returned to post-carrageenan levels 60 Tracey maier stripper after minocycline administration, as indicated by a mean withdrawal threshold of 4. Abstract We have previously reported that NADPH oxidase 2 Nox2 Tracey maier stripper up-regulated in spinal cord microglia after spinal nerve injury, demonstrating that it is critical for microglia activation and subsequent pain hypersensitivity. Fluorescein isothiocyanate-conjugated donkey anti-mouse antibody purified, 1: Rats treated with vehicle showed no significant difference from baseline.